Fig. 8.
(A) SDS-PAGE (12%) for IPTG induced E. coli BL21(DE3) pLysS pET 28 a (+) harboring IFNα cDNA inclusion bodies protein (Lane 2) and solubilized IFNα inclusion bodies (Lanes 3-7). (B) Elution profile of C. dromedarius IFNα after nickel affinity chromatography. Column flow rate was adjusted to be 3 mL/5 min. (C) SDS-PAGE (12%) for nickel affinity purified camel IFNα protein (Lane 2). Lanes 1 in Panel A and B represent pre-stained molecular weight protein markers.