Agarose gel electrophoresis results of (A) Purified vector and insert fragments and their templates (B) the sequenced right his-AGR2-DsRed plasmids extracted from transformed E.coli. Line 1 to 4 were respectively pDsRed-Monomer-N1 plasmid, DsRed insert fragment PCR product, pMAL-his-AGR2 plasmid, his-AGR2 vector fragment PCR product, pMAL-his-AGR2-DsRed plasmid. L represented DNA Leader.