Effect of salicyliden acylhydrazide on Desulfovibrio spp. biofilm formation. Desulfovibrio spp. was grown in lactate sulfate medium for 72 h at 37°C and pH 7. Various concentrations (5, 40, 80 and 100 µM) of salicylidene acylhydrazide were added. OD was measured at 590nm. Significant reduction in biofilm formation was observed at 40, 80 and 100 µM (p˂0.001). Error bars represent standard deviation (n=3) and * = significantly different.