Effect of miR-1468 on CREB1 protein expression level in pituitary cells of Yanbian yellow cattle. (A) The relative expression level of CREB1 protein in pituitary cells transfected with miR-1468 minics. Mimics (miR-1468-mi group) and mimics reference substance (NC group) of miR-1468 were transfected into pituitary cells of Yanbian yellow cattle, with three replicates in each group. Compared with NC group, the column marking** showed extremely significant difference (P<0.01); (B) The relative expression level of CREB1 protein in pituitary cells transfected with miR-1468 inhibitor. Inhibitor (miR-1468-in group) and inhibitor reference substance (iNC group) of miR-1468 were transfected into pituitary cells of Yanbian yellow cattle, with three replicates in each group. Compared with iNC group, the column marking* showed significant difference (P<0.05). β-actin was used as an internal reference. Electrophoresis of the CREB1 and β-actin gene fragment was from two separate gels.