Germline transgene transmission in offspring. Panel A shows that the offspring (litter No. 2), which were generated by mating hGFAP-DsRed male transgenic founders with wild-type female Guangxi Bama mini-pigs, were clinically healthy and developed normally. The exogenous transgene fragments transmitted in the genome of these offspring were confirmed by PCR genotyping (B). However, the astrocyte-specific expression of DsRed in the transgenic founders was absent among various brain regions including the cortex, olfactory bulb, corpus striatum, hippocampus, cerebellum, and spinal cord, indicating that the inherited transgene was not expressed in the offspring (C). (Scale bars = 100 μm).