Establishment of transgenic mice and stable inheritance. (A) Protocol for transgenic mice generation. The healthy adult male mice were chosen for testicle injection, and after 5 days one treated male and three healthy non-pregnant adult female mice were put in one cage. The resulting positive F1 males were mated with female mice after sexual maturity to produce F2 generation. (B) DNA PCR assay of F1 generation tail tissue. M: DL5000bp marker; 1-9, experimental group; 10: negative control; 11: positive control. (C). Western blot assay results of F1 generation. 1-9: the experimental group. (D). Number of F1 and F2 with SCD1 expression in transgenic mice. (E). Expression of SCD1 increased significantly in longissimus dorsi and crureus of F1 transgenic mice. Error bars represent mean value ± SEM. (* p < 0.05, ** p < 0.01).(F). Expression of SCD1 increased significantly in Longissimus dorsi and Crureus of F2 transgenic mice. Error bars represent mean value ± SEM. (* p < 0.05, ** p < 0.01).