Sensitivity tests for real-time RT-PCR and DARPM. Serial 10-fold dilutions of CDV cDNA and CDV virus culture supernatant (CDV cDNA: 6.23×107 copies·μL-1 to 6.23×100 copies·μL-1, CDV virus culture supernatant: 2.28×105 copies·μL-1 to 2.28×100 copies·μL-1) were detected. A minimum of 9.56×101 copies of CDV cDNA and 7.77×101 copies of CDV virus culture supernatant were detected. A: 1-8: Equal dilutions of CDV cDNA at 10-1–10-8, 9: negative control; B: 1–6: CDV virus culture supernatant at 10-1–10-6, 7: negative control.