Root induction and acclimatization of plants in soil under controlled conditions; (A) Plantlets developed roots on half-MS media with IBA 3 mgL-1 (bottom view); (B) Plants transferred to soil to acclimatize under controlled long day conditions at 25 oC and kept covered to ensure high humidity. Scale bar = 3cm; B) Positive expressing NtSAT4 transgenic plantlets transferred to soil to acclimatize under controlled long day conditions at 25 oC and high humidity. Scale bar = 3cm; (C) Developed transgenic plant with early floral buds covered with perforated plastic bags to ensure self fertilization and seed setting.