Knocking down IRK1 causes dampening of evening anticipation peak. Male flies aged 5-7 days raised on normal food and 12:12 LD cycle were put in DAMS under DD: LL condition to observe the circadian rhythm. The flies were left for 24 hrs in DAMS to acclimatize to the environment. First three were continuous dark and last three days were continuously light exposed. The average group activities were plotted as eduction graphs using FaasX. On the x-axis time is plotted in hours and on the y-axis are the average group activities of flies in one channel. A, the control flies with normal evening and morning anticipation peaks as indicated by the blue and red arrows, respectively. B, the diminishing evening peak (blue arrow) and the normal morning peak (red arrow).