Figure 3

Amino acid sequences of the recombinant proteins boPAG1 and Trx-boPAG1 as compared to a prototype from Genbank (BC134743). Identical positions in the alignment are shown against a dark background. Missing sequences in non-homologous regions are indicated by dashes. For the Genbank sequence, the export signal (single horizontal line) and propeptide (double horizontal line) are marked. The export signal and propeptide are absent in the recombinant proteins. Thioredoxin is marked with a line under the alignment as well as a protein purification tag (10xHis). Aspartic acid-372 in BC134743 is replaced by asparagine in recombinant proteins because a mini-gene obtained in this work differs in this amino acid.