Agarose gel electrophoresis showed amplified fragment of 1282bp of B. melitensis bvfA gene. Lane L: molecular marker (100bp- 1500bp), lane N: negative control and lane 1 and 4: positive B.
melitensis bvfA gene and lanes 2, 3, 5, and 6-10: negative for B. melitensis bvfA
gene.