Expression pattern of crucian carp Wap65 mRNA in different experiment. a, Relative expression of Wap65 in crucian carp determined by qRTPCR. The Y-axis represents normalized relative expression values of Wap65. Tissue RNA samples are labeled along the X-axis; b, Fold induction of crucian carp Wap65 gene after heat treatment in the liver. Relative Wap65 expression was expressed as fold change over control samples taken at the same time interval as normalized to change in expression in the β-action control (n=3, *P<0.05).