Representative immunoblots showing expression of β-actin, total Akt and phosphorylated Akt (Thr) along with graphic representation of p-Akt/Akt relative expression (A, B). In the first cycle of differentiation to adipocytes, immunoblots show an upregulation of p-Akt in miR-22-3p transfected cells in comparison to control and negative control groups (C, D). Representative immunoblots and graph following the third cycle of differentiation also indicate an upregulation of p-Akt (Thr) at this stage. Experiments were performed in triplicates and the average percentage values were calculated. The data is presented as mean ± S.D. The results were not statistically significant.