Number of pachytene spermatocytes following 10ρg (A), 1ηg (B) and 1µg (C) doses after 2, 4, 6, 8, 10 and 12 days of alone kisspeptin treatment. Significant decrease occurred in pachytene spermatocytes at 1ηg and 1µg kisspeptin doses after 4 days of treatment, which continued to 12 days. No effect was observed at 10ρg kisspeptin dose throughout the experiment. Values are expressed as mean ± SE (n=7). *p< 0.05, **p< 0.01, ***p < 0.001.