A, Sequence alignment by BLAST; B, 12 % Acrylamide: bisacrylamide electrophoresis photograph showing DNA fragment containing codon 13 after restriction. Sample lanes 1-7 and 9, wild type; lane 8, NHL (heterozygous mutant); and lane M is 50 bp DNA marker (Fermentas); C, Sequencing shwoing GGC (glycine) to GAC (aspartic acid).