SDS-PAGE results for the components of type D8901 botulinum in 40% ammonium sulfate. 1, 100% supernatant40µĺ+β-ME; 2, 100% supernatant40µĺ-β-ME; 3, 40% supernatant15µĺ+β-ME; 4, 40% supernatant15µĺ-β-ME; 5-9, 40% supernatant 15µĺ-β-ME.