2% agarose gel showing amplifications of ORF1 and ORF 2 of aHEV genome in bile samples. (A) Helicase (ORF1) amplification (186 bp, arrow); Lane 1: PT7B; lane: PT9B; lane PT10B; lane 4: PT11B; lane 5: PT12B; lane 6: PT13B; lane 7: PT14B; lane 8: PT16B; lane 9: positive control; lane 10: 1 kb plus DNA marker; (B) ORF2 amplification (280 bp, arrow); Lane 1: PT7B; lane 2: PT9B; lane PT10B; lane 4: PT11B; lane 5: PT12B; lane 6: PT13B; lane 7: PT14B; lane 8: PT16B; lane 9: positive control; lane 10: negative control; M: 1 kb plus DNA marker.