Optimizing the temperature of the RT-LAMP reaction. RT-LAMP was used to amplify PRRSV containing samples. The products of the RT-LAMP reactions were visualized by (A) 1% agarose gel electrophoresis. (B) UV transillumination, and (C) color change using SYBR Green I dye. Lanes: M; DL2000 Marker (A only), 1-5; 59, 61, 63, 65, and 67 C, respectively.