Phylogenetic tree: The tree was constructed using amino acid sequence of amylase from Geobacillus SBS-4S and reported sequences from NCBI GenBank. The name at the end of each branch present the bacterial strain with accession number from which the amylase sequence was originated. Clades A, B and C present the GH family 13, 77 and 70, respectively. The tree was constructed at a genetic distance of 0.5 using Mega 4 software.