Alignment of HA amino acid sequences for the isolated strains in comparison to the reference one in GenBank using “A-Quail-HongKong-G1-97”, A/quail/Egypt/113413v/2011 and A/chicken/Egypt/ME543v/2016. HA1 region of H9 (AIV) was highlighted as yellow color, encoding amino acids (1-320). While HA2 region of H9 (AIV) was highlighted as green color, encoding amino acids (321-503). Cleavage site were marked as red color, including amino acids (315 to 323). The two mutated glycosylation sites were marked as blue color, including sites (188-191), and (200-203).