Fig. 4.

Real time PCR validation of differentially expressed genes and long nocoding RNA in dominant and indominant follicles. Abundance of target genes was normalized relative to abundance of GAPDH gene. Bars in each panel represent the mean ± standard error (sample number = 3 and 3 parallel repetition per sample), * P < 0.05; ** P < 0.01; *** P < 0.001. BMPR1B, IGFBP5, PTGS-2, BMP2, IGFBP5, BMPR1B, LOC102181730, LOC102184274, LOC102183322 were significant up-regulated expressed in NF in compare with DF; BAX, BCL2, INHA, INSIG were significant up-regulated expressed in DF in compare with NF, which identified from RNA-seq.