Identication and generation of transgenic goat. (A) Protocol for transgenic goat production. The healthy adult male goat were chosen for testicle injection twice, and after 47 days, one treated male and three healthy non-pregnant adult female goat were mated to produce F1 generation. (B) DNA PCR assay of tail tissue in F1 generation. M, DL5000 bp marker; 1-7, experimental postive group; 8, negative control; 9, positive control. (C) Western blot assay results of F1 generation. 1-7, the experimental group. (D) Number of transgenic F1 goat with SCD1 expression.