Cloning of HCV3a E2 gene with NdeI and HindIII restriction sites. (a) Lanes 1-4, Amplified gene E2 with NdeI and HindIII restriction sites. (b) Lane 1, Recombinant plasmid pTG19-E2; Lane 2, pET21a. (c) Lanes 1-3, Restricted pTG19-E2; Lanes 4-7, Restricted pET21a; Lane M, GeneRulerTMDNA ladder mix (Fermentas Cat # SM0331).