hAD helps to detect extracellular AGR2 internalization in NIH3T3 cell line which does not have internal AGR2 expression as well as in A549 which express internal AGR2. Cells were treated with different concentrations of hAD for 24h. Internalization of hAD was shown by (A) Western blot analysis. (B) Immunofluorescence assay. Scale bar, 25μm.