Expression and localisation of MRP-1 in prostate cancer cells. (A) The mRNA levels of ABCC1 in PC-3/Wt and PC-3/Res cells were obtained by real time RT-PCR and normalised to beta 2 microglobulin (B2M) expression (B) ABCC1 protein expression was analysed by Western blot using anti-MRP-1 antibody. Ponceau S staining is shown as loading control (C) Immunofluorescence analysis of ABCC1 protein in PC-3/Wt (upper panel) and PC-3/Res cells (lower panels). Cells were fixed and immunolabelled for ABCC1 (red) using anti-MRP-1 antibody. Merged images, including nuclei (stained with DAPI), were assembled using Adobe Photoshop CS6 Software. Bar, 20µm.