Construction and cloning of his-AGR2-DsRed plasmid. (A) Flowchart for the construction of his-AGR2-DsRed plasmid. (B) A schematic representation of his-AGR2-DsRed plasmid map. Essential elements include a poly-histidine tag, human AGR2 gene without signal peptide, a (GGGS)3 flexible linker, DsRed monomer gene as well as genes encoding the LacI repressor (lacR), ampicillin resistance (AmpR) and origins of replication.