The Sertoli cells proliferation was inhibited by DUSP4, PDK4 or FKBP1B. The DUSP4, PDK4 or FKBP1B recombinant plasmid was transfected into Sertoli cells. A, the mRNA expression levels of DUSP4, PDK4 and FKBP1B; B, Western blot detection results for DUSP4, PDK4 and FKBP1B; C, MTT assay results after recombinant plasmid overexpression (**p<0.01).