Western blot analysis of VEGF protein expression in G-Rh2 treated embryos. Western blot of Vegf expression in 72 hpf zebrafish embryos treated with different concentrations of G-Rh2 or 0.1% DMSO (vehicle control). β-Actin was used as loading control. Different letters (a–d) above the columns indicate significant differences (P < 0.05).