Effect of Temperature and pH on the AMYSBS activity. A, Effect of temperature. The activity was done at various temperatures ranging from 40 to 70°C in 50 mM Tris-HCl buffer (pH 8) using 0.4% starch as substrate. B, Effect of pH on the activity of AMYSBS. The activity was examined in 50 mM of each of acetate buffer (4-5), phosphate buffer (6-7), Tris-HCl buffer (8-9) and Glycine/NaOH (10-11) using 0.4% starch as substrate at 55°C.