Figure 3

SDS-PAGE analysis of the pCold II-INPM2 protein expression. A, transformed pG-TF2 expression strains and induced with 0.5mM IPTG; B, transformed pG-TF2 expression strains and induced with 0.5mM IPTG. Lane A, non-induced crude; Lane B, Induced crude; LaneB1-B4, Induced crude; Lane C, Supernatant of lysate; Lane D, Precipitation of lysate. MK, Molecular weight marker.