MSCCM inhibits the expression of TGF-β1 and prevents the NF-κB activation in MRC-5 cells after irradiation. A, D and G, TGF-β1, IFN-γ and NF-κB/p65 levels were determined by ELISA in lysate of MRC-5 cells in control cell group (Con), single-irradiated MRC-5 cell group (IR), and irradiation + MSCCM treated MRC-5 cell group (IR+MSCCM). B and E, the mRNA levels of TGF-β1 and IFN-γ were examined by q-PCR in different treatment groups. C, F, H and I, protein levels of TGF-β1, IFN-γ, NF-κB/p65 and IκB were detected by western blot. Data are expressed as the mean ± SD (n=5). **p<0.01, ***p<0.001.