Quantitative determination of planktonic and loosely attached cells of three S. Gallinarum strains (SG 119/ Pak, SG421/India and SG 2a 189/China) using test tube assay via CV staining method. Briefly bacteria were grown in test tubes for 3, 5 and 7 days. Each set was taken out after respectives time period, cells were centrifuges and stained with 0.1% CV stain OD580 [/] was measured. Results are replica of three independent experiment. One way ANOVA followed by post hoc Tukey test was used to analyze results in SPSS (Version 13.0). Bars with no common superscript are significantly different (p<0.05).