Agarose gel electrophoresis (a) PCR amplification of HCV3a E2 gene. Lanes 1-6, Amplified 1056bp E2 gene whereas lane 7 is E2 no template control. (b) Restriction analysis of pTG19-E2 with restriction enzymes EcoRI and BamHI. Lane 1, Single restriction of pTG19-E2 with restriction enzyme EcoRI. Lane 2, Restriction of pTG19-E2 with BamHI that cuts at both ends of the insert; 2.8kb digested vector releasing 1077bp band of E2 gene; Lane M, GeneRulerTMDNA ladder mix (Fermentas Cat # SM0331).