Apoptosis in pre-ovulatory follicles of C. gariepinus. A and C, arrow shows pyknosis, irreversible condensation of chromatin in the nucleus and condensation of cytoplasm of oocytes undergoing apoptosis; B, pyknosis followed by the fragmentation of the nucleus. (karyorhexis) and the budding phenomenon detected in pre-ovulatory oocytes of C. gariepinus. Arrow shows the budding phenomenon. P, pyknosis; K, karyorhexis; Ap, apoptotic body. Scale bar, 200µm. Stain: hematoxylin & eosin.