SDS-PAGE analysis of the pCold II-INPM2 protein expression. A, transformed E. coli BL21 (DE3) cells and induced with 0.5 mM IPTG; B, transformed E. coli BL21 (DE3) cells and induced with 0.1mM IPTG. Lane A, non-induced crude; Lane B, Induced crude; LaneB1-B4, Induced crude; Lane C, Supernatant of lysate; Lane D, Precipitation of lysate. MK, Molecular weight marker.