Fig. 4.

Purified AChE of Tribolium castaneum on 12% SDS-PAGE and western blot. A band of 35kDa is visible. Lane 1, marker; Lane 2, marker in 1% SDS-PAGE; Lane 3, crude extract of insect; Lane 4, purified fraction from gel filtration and preparative PAGE; Lane 5, immuno-blot of purified protein.