SDS-PAGE (12%) for IPTG induced E. coli BL21(DE3) pLysS pET28 a (+) harboring C. dromedarius IFNα cDNA (Lane 2), IPTG induced culture supernatant (Lanes 3-5), and IPTG induced culture inclusion bodies (Lanes 6-8). (B) SDS-PAGE (12%) for IPTG induced culture (Lanes 1-3) and western blotting analysis with 6x-His-Tag monoclonal antibody (1:1000 dilution) for IPTG induced culture (Lanes 6-8). Lanes 1 (Panel A), 4 (Panel B) and 5 (Panel C) represent pre-stained molecular weight protein markers.