The RE second round PCR products; Lanes 1 to 8 on left represent PCR amplification using first round PCR amplified without human genomic DNA; Lane 9, negative control; Lanes 1-8 on the right represent PCR amplification using first round PCR amplified with added human genomic DNA; Lane 9, negative control; M, DNA marker (SolisBiodyne 1Kb DNA ladder, cat# DM010-R500).