The impact of L-cysteine and vitamin E supplemented diluents on freeze-thawed spermatozoa parameters in Achai × Jersey bulls (Mean±SEM). Control diluent containing no additives; L-c-1 diluent containing 5 mmol of L-cysteine; L-c-2 diluent containing 7.5 mmol of L-cysteine; L-c-3 containing 10 mmol of L-cysteine; Vit-E-1 containing 1.2 mmol of vitamin E; Vit-E-2 containing 2.4 mmol of vitamin E; Vit-E-3 containing 4.8 mmol of vitamin E. AI, acrosomal integrity; MMP, mitochondria membrane potential; SV, sperm viability; different letters show significant difference (P<0.05).