A, All cells at passage 4 showing flattened and spindle-shaped morphology, A) cultured with FBS, 100X, B) culture with AES, 100X (AES) and C) cultured with ABS, 100X (ABS). No detachment was observed under any serum group. B, Viability of ATF’s assessed by trypan blue stain method showed no significant difference and data was expressed as Mean±SEM by keeping level of significance ≤0.05.