Ion exchange chromatography of placental proteins of Bubalus bubalis isolated at pH 9.5. 15 mg of protein was loaded onto a column packed with DEAE-Sepharose (0.8x15cm); using a salt gradient 0.01-0.5M NaCl in seat shows dot blot analysis of peak fraction indicating presence of bPL. Fractions from peak No. 1 showed immunogenic activity against placental lactogen antibody (Abcam, USA) (Fraction No. 28-48).