Figure 5

Reconstructed GV oocytes with somatic cells after 17 hr. of maturation. Extruded large-sized PBs (A, B), DNA was stained with Hoechst 33342 where the oocyte had scattered chromosomes (C), DNA was stained haematoxyline where the oocyte had interphase-like nucleus (D), the oocyte had partially condensed chromosomes (E) the oocyte had uneven distribution of chromatin (micronuclei)