(A) Bovine skeletal muscle satellite cells (bSMSCs) cultured in vitro; primitive bSMSCs were rounded and had strong refraction; (B) The fast-adhered cells were fibroblasts and the bSMSCs were round and suspended; (C) Two days later, the bSMSCs were spindles and proliferating rapidly; (D, E, F) After two cell passages and differential adhesion, the cells were confluent and became homogeneous in their appearance.