The effect of pH on (a) POR and (b) PDC specific activities of the purified enzyme from T. kodakaraensis. The open boxes indicate the pH dependent experiments with sodium phosphate buffer (pH 6.5, 7.0, 7.5 and 8.0), the closed boxes represent the experiments with Tris HCl buffer (pH 8.0, 8.5 and 9.0), open circle represent the reactions with CHES buffer (pH 8.5, 9.0, 9.5 and 10.0) and closed circles exhibit reactions with CAPS buffer (pH 9.5, 10.0, 10.5 and 11.0). Effect of temperature on (c) POR and (d) PDC specific activities of the purified enzyme from T. kodakaraensis. The assay was performed as described in Materials and method section.