Promoter activity analysis of the Almyomaker gene. A, the structure of the Almyomaker promoter and 5’UTR region. Five recombinant plasmids were denoted myomaker-p1 (-1822 to +267), myomaker-p2 (-1215 to +267), myomaker-p3 (-943 to +267), myomaker-p4 (-168 to +267) and myomaker-p5 (-34 to +267). B and C, Transcriptional activity of the Almyomaker promoter. These plasmids were transfected along with the transcription factors MyoD1 (B) and MyoD2 (C) into HEK 293T cells. Dual-luciferase activity was driven by the Almyomaker promoter upon the transfection of pcDNA3.1-MyoD1, pcDNA3.1-MyoD2 or pcDNA3.1 into HEK 293T cells. Data are presented as the means of three replicates ± SE. Different letters indicate significant differences (p < 0.05).