Fig. 5.

 Western blot analysis of the specificity of polyclonal antibodies to BmARM-like protein. Samples were separated by 12% SDS-PAGE under reducing conditions. M marker; 1,2,3 SDS-PAGE; 1’,2’,3’ Western blotting; lane 1,1’ lysate from E.coli without induction, lane 2,2’ lysate from E.coli following induction with IPTG, lane 3,3’ total protein extracted from silkworm. 71 kDa band in silkworm and 31.1 kDa band in E.coli were correspond to BmARM-like protein.