Expression of five muscle-specific genes in the anterior and posterior muscles to the dorsal fin in both WT and imbs mutant at five developmental stages. A, C, E, G and I represented the expression of myod, myf5, myog, mef2ca and sox6 in the anterior muscles of the dorsal fin at the five stages (15 dpf, 30 dpf, 45 dpf, 60 dpf and 75 dpf), respectively. B, D, F, H and J showed the expression of the five muscle-specific genes in the posterior muscles of the dorsal fin at five different stages. The gene expression was normalized to the expression level of the reference gene gapdh. Data are expressed as mean ± SD of 2-△△CT (n=3). *, P<0.05; **, P<0.01; ***, P<0.001; n.s., no significance.