Effect of 5µM concentration (A), 10µM concentration (B) and 15µM concentration (C) of trypsin, proteinase K and chymotrypsin on Desulfovibrio spp. biofilm formation. Desulfovibrio spp. was grown in lactate sulfate medium at 37°C and pH 7 for 72 h. OD was measured at 590 nm in A and B. Significant reduction in biofilm formation was observed in C using all compounds (p˂0.01). Error bars represent standard deviation (n=3) and * is significantly different.