Influence of HGQ on the senescence of N2 nematodes. Synchronized nematodes were incubated at 20°C in 96 wells plates with or without HGQ at the indicated concentrations, and the representative Kaplan-Meier survial curves are shown from three independent experiments with about 100 animals in each group (A). The fluorescence intensity of lipofuscin in adult nematodes was detected from day 1 to day 10 by a microplate reader at 355 nm excitation and 460 nm emission, and data are shown as mean ± SD of ten parallel wells with 100–150 nematodes per concentration (B).