Evaluation of DNA damage in different classes of active smokers addicted to Charas and snuff. Quantification analysis of the levels of DNA damage in five different classes of active smokers addicted to charas and snuff. The numbers of cell’s nuclei which satisfied the criteria for class 0 were very few in all three groups of active smokers. The total TCS values were gradually increased in different classes of active smokers. The highest number of distorted nuclei with maximum DNA damage was found in charas-addicted smokers. However, this number was low in active smokers who were not addicted to charas or snuff.